Find out the amino acid sequence of a decapeptide

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Reference no: EM13188218

A protein biochemist attempted to determine the amino acid sequence of a decapeptide. Use the results fromt he trypsinm chymotrypsin, and cyanogen bromide treatments to suggest the amino acid sequence.

Trypsin digestion gave two fragments

T1: Ala, Arg, Phe, Pro, Thr, Trp, Tyr

T2: Lys, Met, Val

Chymotrpysin digestion gave three fragments with the following residues

CT1: Ala, Arg, Phe, Pro

CT2: thr, Trp

CT3: Lys, Met, Tyr, Val

N terminal analysis of the CT1 tetrapeptide indicated that the N-terminal amino acid was Ala. The cyanogen bromide gave only one single amino acid, methionine, and a nonapeptide. wWhat is a possible sequence of the decapeptide? Use three letter abbreviations

Reference no: EM13188218

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