Reference no: EM132657639

In addition to plate counts, you also set up a dilution-to-extinction experiment using the same medium as for the agar plates, albeit without agar. After extracting cells from each sample type (surface lake water, surface soils, and subsurface soils), you inoculated the wells of each dilution-to-extinction experiments to cell density of 10 cells well-1. For each sample type, you inoculated 3 × 96-well plates. You incubated the samples at ambient temperature for three weeks and screened each 96-well plate for growth using a flow cytometer. You obtained the following data:

Environment Total number of wells with growth across all three 96 well plates
Surface lake water 42
Surface soil 137
Subsurface soil 252

Which environmental sample produced the highest number of pure cultures? Show your work (1 point) and provide the correct answer (1 point).