Find final concentration of iptg in the bacterial culture

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Reference no: EM1393913

1. In this lab, you will add 4uL of 1M IPTG to 4mL of bacterial culture. What is the final concentration of IPTG in the bacterial culture?

4 uL = 0.004 ml
Mf = Mi x Vi / Vf
Mf = 1 x 0.004 / 4 = 0.001 M

2. Predict how many bands of DNA you will see in the double digest if
a. you have GST only?
One (circle)

b. you have GST-Cherry?
Two

3. The teacher was not paying attention and put the red lead into the black slot and the black lead into the red slot of the power supply. This reverses the current as shown below. In this incorrect orientation, where will the DNA end up (at the top of the gel, at the bottom, stuck in the wells, dissolved, etc)?

Reference no: EM1393913

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