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1. In this lab, you will add 4uL of 1M IPTG to 4mL of bacterial culture. What is the final concentration of IPTG in the bacterial culture?
4 uL = 0.004 mlMf = Mi x Vi / VfMf = 1 x 0.004 / 4 = 0.001 M
2. Predict how many bands of DNA you will see in the double digest ifa. you have GST only?One (circle)
b. you have GST-Cherry?Two
3. The teacher was not paying attention and put the red lead into the black slot and the black lead into the red slot of the power supply. This reverses the current as shown below. In this incorrect orientation, where will the DNA end up (at the top of the gel, at the bottom, stuck in the wells, dissolved, etc)?
Determine which well-known goat feeding strategy combined with which errors in the design and execution of digestion trials led some animal nutritionists to believe that goats
Think about an artificial animal cell which contains 0.3 mmol/L NaCl (MW: 58.5 g/mol) and 0.01 mmol/L glucose (MW: 180 g/mol) in the cytoplasm.
Hereditary fructose interolance is a rare recessive disorder that is caused through a mutation in the aldolase B gene. As a result of this disorder individuals may have all of the following symptoms except:
Although physiologically distinct, chemolithotrophs and chemoorganotrophs share a number of features with respect to the production of ATP.
RNA was extracted from plasma cells (PC) of wildtype (WT) mice and plasma cells (PC) from mice expressing a Myc short hairpin RNA.
Earlier in 2010, the Pacific island nation of Samoa decided to switch from driving on the right to driving on the left. Why may these cause difficulties? Why is it complicated for a cell to change codon usage, say, switching a "stop" codon to a trypt..
Determine which of the following statements would be inaccurate regarding the discovery of the DNA molecule?
What is the possibility that their first child will have attached earlobes. which among the conditions or substances do ALL archaeans need for survival and reproduction.
The endosymbiosis theory discusses that mitochondria and chloroplasts were originally prokaryotic cells that were engulfed by ancestors of current day eukaryotic cells.
Assume that two QTL affect the weight of a dog breed. Each locus has two alleles such that each dose of the "2" form of an allele adds 5 pounds to the base weight of 60 pounds,
There are several examples of eukaryotic cells. Algae, Fungi, Plants, and Animals are all comprises of eukaryotic cells.Select either a plant or an animal cell.
The Kingdom Fungi contains members with saprobic, parasitic and mutualistic modes of nutritiom. How do these types of nutrition relate to the ecological and economiv importace of this group.
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