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Question: Assume you have identified a mutant E. coli strain that cannot synthesize histidine (his-). To determine the location of the his- mutation on the E. coli chromosome,interrupted mating experiment was performed with 5 different Hfr strains. The results were as follows (transfer time in parenthesis):Hfr A bio (4) glu 20 his 27 cys 37 tyr 45Hfr B xyl 6 met 18 tyr 24 cys 32 his 42Hfr C his 3 cys 13 tyr 21 met 27 xyl 39Hfr D xyl 7 thr 25 lac 40 bio 48 glu 62Hfr E his 4 glu 11 bio 27 lac 35 thr 50
On the map below of the circular E. coli chromosome, indicate:a. relative location of each geneb. position where the sex factor is integrated in each of the five Hfr strainsc. the direction of chromosome transfer for each Hfr (indicate with arrow)
Compared to a carbon atom with only single covalent bonds, a carbon with a double bond 1 Shares fewer electron pairs with other atoms two Is more flexible in the spatial arrangement.
Using Mendelian genetics you can find out the likelihood of your eye color, your hair color, some things that fall in these lines, but at the age of 25 I would like to know how I can determine when I will have a head full of white hair.
Explain the pattern of uterine involution. What do these abbreviations mean: U/U, 1/U, U/2. Explain the correct way to massage the uterine fundus.
Discuss the difference between an antiseptic and a disinfectant and determine what are chemotherapeutic agents?
In forensic analysis using STR systems, up to 16 different loci can be tested in a single PCR reaction. Why is it important for forensic analysis that each locus being tested is present on a different chromosome? Ideas are justified.
What are the genotypes of parent orange-fruited plants. Within the two groups defined by bone mass smaller skeletons have bones with evidence of epiphyseal plates should be. Give the age group and gender of the individuals in this fine.
If the mutation is in mitochrondrial DNA, will the four haploid cells probably be all Pyr-, all Pyr+, or a combination? What if the mutation is in nuclear DNA. Why is mitochondrial function critical for growing on pyruvate.
Sketch another graph showing the response to selection if the heritability, h2, of pesticide resistance is 0.8. Illustrate an experiment that you could use to measure how this population of beetles responds to the selection that occurs when the popul..
Suppose if ICF (K+) ion concentration is 165 mM, what ECF (K+) concentration is required for the condition of no net passive flux of K+ across the membrane at Vm = - 66 mV?
The replication cycles of bacteriophages and animal viruses have many similarities. Discuss similarities and differences of the characteristics of prophages vs proviruses.
Discuss how to figure out a cDNA sequence from an amino acid sequence? This is usually in context of determining an appropriate oligonucleotide probe.
Compute the change in energy of an atom that emits a photon of wavelength 2.21 meters. (Planck's constant is 6.626 x 10-34 joule seconds; the speed of light is 2.998 x 108 m/s)
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