Find cell density of cell suspension

Assignment Help Biology

Reference no: EM1385935

Question: In order to perform a fluorescence experiment, you need to seed a 4-well chamber slide with 60,000 cells in each well. To prepare for the cell count, you transferred 20 uL of cell suspension into 30 uL of trypan blue. You calculated 19, 12, 25, 29 and 11 cells in 5 large boxes using the Neubauer hemacytometer. Based on this information, what is the cell density (cells per mL) of your cell suspension? How much cell suspension will you need to transfer roughly 60,000 cells into to each well?

Reference no: EM1385935

Questions Cloud

Describe the company in terms of size : Using the case scenario above, provide an assessment of the current business. Describe the company in terms of size, type of business, etc. Explain why you think an HRIS is needed.

How to choose optimal location of pipeline for oil field : A Consultant is to lay oil pipeline running east to west through the oil field of n wells. From each well spur pipeline is to be connected directly to main pipeline along shortest path.

Maximum variance of the raw score population : What maximum variance of the raw score population could you have and still be able to reject the null hypothesis of μ = 20 (α = .05), if the sample size is 100 and the sample mean is 22?

Assuming that the current turnover and profits of both : Assuming that the current turnover and profits of both the units are comparable, compare the relative benefits and limitations of Materials Requirement Planning (MRP) for these two businesses.

Find cell density of cell suspension : In order to perform a fluorescence experiment, you need to seed a 4-well chamber slide with 60,000 cells in each well. To prepare for the cell count, you transferred twenty uL of cell suspension into thirty uL of trypan blue.

Challenges that users face in building information systems : Discuss major challenges that typically users face in building and/or using information systems

Current event and contemporary social issue : Determine a current event or contemporary social issue that you are interested in analyzing using the critical thinking method. Your issue must be broad enough to sustain deep philosophical analysis

Determine the standard error of the proportion : 1) There are 500 employees in a firm, 45% are female. A sample of 60 employees is selected randomly. a) Determine the standard error of the proportion.

What is john and his employees productivity : What is their productivity? John and his employees have discusses redesigning the process to improve efficiency. If they can increase the rate to 125 per day, what will be their new productivity?

User Account

All Pages