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Question: In order to perform a fluorescence experiment, you need to seed a 4-well chamber slide with 60,000 cells in each well. To prepare for the cell count, you transferred 20 uL of cell suspension into 30 uL of trypan blue. You calculated 19, 12, 25, 29 and 11 cells in 5 large boxes using the Neubauer hemacytometer. Based on this information, what is the cell density (cells per mL) of your cell suspension? How much cell suspension will you need to transfer roughly 60,000 cells into to each well?
Speculate on and/or research potential mechanisms which would afford the improved processing. Speculate on and/or research potential mechanisms.
Assume y ou are analyzing a dominant allele in fruit flies that mutates to a recessive allele at the relatively high rate of 10 -6 per generation.
Assume you isolate the enzyme that synthesizes folic acid in bacteria and conduct few enzyme kinetics experiments. You discover, not surprisingly, that sulfa drugs inhibit enzyme's activity
Describe the etiology and genetics of the disease phenylketonuria (PKU). Phenylketonuria (PKU) refers to a disease found in the humans in which the affected individual does not have enzyme phenylalanine hydroxylase.
Think about the phenomenon of bacterial resistance to antibiotics. How did the combination of human advances and basic evolutionary principles lead to this condition?
A certain instant camera determines the distance to subject by sending out a sound wave and measuring the time required for the wave echo to return to the camera. How long could it take the sound wave to return to the camera if the subject were 2.68 ..
Explain specifically how irradiation using the following methods destroys microbes provides an examples(s) of materials that can be sterilized by each of the techniques below.
Angel is a biologist studying Lake Ecosystem in which leaf-eating insects are consumed by fish, which are consumed by snakes, which are consumed by hawks. In this case, how would you categorize a fish.
G3-15ml frozen bacterial pellet was added to the breaking buffer and a freeze-thaw procedure mixing the two solutions through sucking up the mixture up and down in the vial was performed.
For the population in Qu 1, we sequence the alleles, Compute the number of SNPs and % segregating sites and Calculate the nucleotide diversity.
Determine which organ system are msot invovled with homeostasis and discuss the role of SNARE in exocytosis.
A cross is made in a pure-breeding green budgie and a pure-breeding albino budgie. What are the genotypes of the parent birds?
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