Reference no: EM131007964 , Length: word count:800
Introductions doesn`t need to be done, i`ll get it done by myself during that time
Just need discussion/conclusion ( up to 800 words)
Detailed Question: We had two practicals in which we had to to extract polyphenol oxidase from mushroom and to to examine the effect of substrate concentration on the rate of the browning reaction.
I have to write a report and include sections such as introduction (talk about enzymatic browning, controls of it, effects of pH etc.), results section (including results table tutor sent us, 2 graphs which i already done and attached), discussion and conclusion.
I have already done introduction section by myself which is gonna be about 1000 words. Already done 2 graphs using the results table tutor sent us. All i need you to do Is just find Vmax and Km for both graphs and write a discussion/conclusion which don't have to be long.
Initial velocity Vo (unit change in absorption per min)
Table shows the data you collected in the PPO practical. The units of initial velocity are absorption/min and concentration is expressed in mM (milli-molar)
| Catechol concentration (mM) |
A (0.5 mL extract) |
B (1 mL extract) |
C (1.5 mL extract) |
D (Denatured enzyme) |
| 1.0 (Exp 4) |
0.057 |
0.08 |
0.087 |
0.004 |
| 2.5 (Exp 3) |
0.114 |
0.15 |
0.215 |
0.002 |
| 5.0 (Exp 2) |
0.121 |
0.223 |
0.354 |
0.004 |
| 10.0 (Exp 1) |
0.191 |
0.305 |
0.415 |
0.001 |
Attachment:- Assignment.rar
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