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A gene regulation lab is trying to identify regulatory elements that control the expression of a liver specific gene called RL in mice. They identify a mouse mutant with reduced expression of this RL hepatic gene. Closer inspection allows them to identify that the mutant mouse with reduced RL gene expression, has no mutations in the RL gene sequence, but has a 50 bp deletion in another gene (ITP gene).
1. Given this information, which of the following would be a potential role for the ITP gene? Provide a 1 sentence explanation for your choice.
i. It encodes for a negative regulator of the RL gene.
ii. It encodes for a positive regulator of the RL gene.
iii. It codes for an enhancer region of the RL gene.
2. Succinctly propose a experimental strategy that can help this lab confirm the predicted role of the ITP gene (from your choice above) on RL gene expression. [Provide name of a strategy/tool you will use, 1 sentence explanation of the experimental design and how will the results either help confirm or reject your prediction]
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