Expression during sex determination in drosophila

Assignment Help Biology
Reference no: EM131325511

To investigate transformer (tra) gene expression during sex determination in Drosophila, you perform in situ hybridization (FISH) and immunostaining. Fill in the table with the results you expect to observe following the example given in the first row.

Experiment

Female embryos

Male embryos

Immunostaining with an antibody to Sxl protein

stain positively

no staining

FISH with a probe to exon 2 of tra



FISH with a probe to exon 3 of tra



Immunostaining with an antibody to Tra protein



Match sex phenotype to each genotype of a transformer mutant fly:

XX: ___________________________;     XY: ________________________

Reference no: EM131325511

Questions Cloud

Discuss the trade-offs involved in selecting values : Plot the rate as a function of p for different values of M, and discuss the trade-offs involved in selecting larger or smaller values of M.
Create two single stranded pieces : Suppose you are performing an experiment in which you must use heat to denature a double helix and create two single stranded pieces. Based on what you know about nucleotide bonding, do you think the nucleotides will all denature at the same time?..
Explain how you would motivate student to perform in class : Assume you are a professor for a college class. The class has 4 students who have different needs: Identify which of Maslow's need(s) each student is trying to fulfill and Explain how you would motivate the student to perform in class
Exemplification as a method of development : Determine exactly what point you are going to make and write it down in precise terms - Choose the best examples that honestly reflect the group or class they represent. Do not pick examples that do not support your thesis. Also, make sure that you..
Expression during sex determination in drosophila : To investigate transformer (tra) gene expression during sex determination in Drosophila, you perform in situ hybridization (FISH) and immunostaining. Fill in the table with the results you expect to observe following the example given in the first..
Path dependent options and path independent options : Explain the advantages and disadvantages of implementing portfolio insurance using stock and puts in comparison to using fiduciary call.
Describes the world in the short run but not in the long run : Most economists believe that classical theory describes the world in the short run but not in the long run. A shift of the money-demand curve from MD2 to MD1 is consistent with which of the following sets of events?
Explain how weather derivatives could be used : Explain how weather derivatives could be used by an electric utility to manage the risk associated with power consumption as affected by the weather.
Discuss the strategies of customer relationship management : IST 309:To help students better understand Customer Relationship Management (CRM) and the e- commerce strategies via a company's website.To find out how effectively Customer Relationship Management contributes to e- commerce with the applications ..

Reviews

Write a Review

Biology Questions & Answers

  Locate a credible website

Select any disease or disorder of your choice. Locate a credible website (hint: government websites such as CDC or NIH, medical association websites such as American Lung Association, Cerebral Palsy Association, etc)   Describe the disease process in..

  Why water is known as polar solvent

Why water is known as polar solvent? How is hydrogen bonding involved in cohesive attraction of water molecules? Water molecules also attract other sorts of molecules, particulary ions.Why is this.

  Identify and exlpain the type of immunity

Identify and exlpain the type of immunity that is present only when a newborn infant is being fed by actively nursing on its mother and ends when nursing ends.

  Is this consumer maximizing his total utility

For a consumer, the marginal utility of good A is 25 and its price is $5. The marginal utility of good B is 60 and its price is $12. The consumer has allocated his entire budget. Is this consumer maximizing his total utility? Explain your answer.

  Describe the development of t-dependent immune response

Describe the development of a T-dependent immune response. Include details of B cells, T cells, and all relevant cytokines.

  Effect of light intensity on the rate of photosynthesis

In an experiment that tested the effect of light intensity on the rate of photosynthesis, we do not run a control tube in theexperimental setup. that is not every good scentific practice isit? why is a control important in an experiment?

  The evolution of disease resistance in domestic turkeys

If we observe that domestic turkeys are less resistant, does that necessarily mean that wild females had been choosing more-resistant males, or are other hypotheses equally plausible? Please include at least one scientific research article to supp..

  Which statement about multiple locus probes is incorrect

Which statement about multiple locus probes is INCORRECT.

  How would you incorporate porter value chain

How would you incorporate Porter's Value Chain and Holistic Marketing Framework when structuring a marketing plan for the example organization.

  Challenges faced by public health system

Recognize the two challenges faced by the public health and also discuss how tools and the guidelines of an epidemiological study is applied in finding the answers and strategies for meeting these challenges.

  Lipid droplets in the endoplasmic reticulum

It is thought that excess lipids in the cell are packaged into lipid droplets in the endoplasmic reticulum. Explain why lipid droplets bare surrounded by a phospholipid monolayer and not a phospholipid bilayer, like other vesicles that bud from the e..

  Effect of an acidic fluid on enzymatic activity

Develop an experiment in which you will test the effect of an acidic fluid on enzymatic activity. To complete this assignment, it may be useful for you to review the Scientific Method Tutorial.

Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd