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Express the protease gene to obtain the enzyme

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Reference no: EM132123845 , Length: 1000 Words

Question - Gene Cloning

a) Please write the steps to clone the protease gene from Bacillus strain whose genome sequence is not known.

b) Express the protease gene to obtain the enzyme in high yield, please plan your protocol according to the host that you choose.

c) What will be your strategy to find the active center of the enzyme.

Reference no: EM132123845

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