Reference no: EM1387037
Question: Palmitate uniformly labeled with the radioisotope 3H to a specific activity of 2.5 x 108 cpm (counts per minute; i.e., amount of radioactivity) per micromole of palmitate is added to mitochondria, which oxidize it to acetyl-CoA. All acetyl-CoA is isolated and hydrolyzed to acetate and CoA. The specific activity of the acetate is found to be 1 x 107 cpm per micromole. I.e., there are 1/25th as many radioactive Hydrogens per molecule of acetate as there was per molecule of palmitate. Is this result consistent with the beta- oxidation pathway? Why? What is the final fate of the missing 3H?
Answer: This is consistent with the pathway because most of the hydrogen is lost from the fatty acids in the form of NADH, FADH2, and H+ ions. Following the dehydrogenation, hydration and dehydrogenation reactions of beta-oxidation, only one labeled hydrogen atom on the acetyl-CoA is cleaved in the following thiolysis step. When the palmitate has undergone seven rounds of oxidation, what is produced is 7 acetyl-CoA, each with one labeled hydrogen, and one acetyl-CoA with 3 labeled hydrogens (the last one does not undergo any modification because the seventh round cleaves the 4 carbon chain into two 2-carbon chains). Adding up the label on all the acetyl-CoA molecules, there are 10 labeled hydrogens on the 8 acetyl-CoA molecules. On average, each acetyl-CoA has an average label of 10/8 = 1.2. Comparing the original label (31 hydrogens) with the final label (1.2 hydrogens), the label is reduced by a factor of 31/1.2 = 25. Thus, the label on the acetyl-CoA is 1/25th of the label on the palmitate.
WHERE I NEED HELP.....I'm having trouble understanding this problem. I understand the process of beta oxidation and why 8 Acetyl CoA's are formed but I'm confused what "radioisoptio 3H" means (where is the radioisotope attatching on the palmitate?) and also the section I italicized in the answer portion. Maybe I don't fully understand the statement since I'm not sure what hydrogen is labeled and where it is being moved to (and why one acetyl CoA has 3 labeled hydrogens while the rest have only 1?)
|
What is the water level h in the tank
: What is the least (non-zero) thickness for the air layer between two flat, and parallel, glass surfaces if the glass is to appear dark while 640 nm light is incident normally? What if the glass is to appear bright.
|
|
Margin of error of the proportion of voters
: Based on our poll what is the margin of error of the proportion of voters favoring the incumbent?
|
|
Psychological research on nonhuman animals
: Discuss how Darwin's theory supported psychological research on nonhuman animals. Give one research question which can only be answered by doing experiments with nonhuman animals in order to shed light on human psychology.
|
|
Explain how are the above characteristics consistent
: Illustrate what characteristics/abilities do you possess or need to develop to be successful in this culture? Explain how are the above characteristics consistent and synergistic with the South Korean culture?
|
|
Explanation of biochemistry problem
: Palmitate uniformly labeled with the radioisotope 3H to a specific activity of 2.5 x 108 cpm per micromole of palmitate is added to mitochondria, which oxidize it to acetyl-CoA.
|
|
By how much does the spring stretch
: A package in an airplane moving horizontally at 275 m/s is dropped while the altitude is 766 meters. How far forward from the spot over which it was dropped does the package land.
|
|
Paddy''s was forced to buy the beer from another source
: Paddy's was forced to buy the beer from another source at $55 per keg. What are Paddy's Article 2 damages? Please explain
|
|
Learning and modified behaviorism
: Tolman initially was attracted to Watsonian behaviorism but eventually developed more sophisticated view of animal and human behavior as purposive and goal-driven. We should focus on Tolman's legacy in research on motivation.
|
|
Explain chain of custody is critical factor for cases
: Think of numerous cases where evidence could potentially be tampered with a cell phone or thumb drive. What can go wrong?
|