Explain why you streaked both cultures

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Reference no: EM133124450

Question 1: Define the terms donor strain and recipient strain and Identify which of the cultures you used were the donor and recipient strains, using justifications and reasoning based on expected results (provided on LJCU). Did conjugation occur in your case (explain why/why not)?

Question 2: Describe the process by which the vio gene was transferred from the donor to the recipient.

Question 3: Explain why you streaked both cultures onto three antibiotic-containing agars at the start of the experiment. Explain how both antibiotics being added together to LB agar will select for transconjugants.

Question 4: Explain why you think you were asked to spread your mixture after two different time periods? How could this timed procedure (if you did more time intervals) help you in the study of the plasmids?

Question 5: From the literature, do you consider conjugation to be the primary method for transfer of antibiotic resistance between bacteria in nature? If not, which method do you consider to be more likely, use scientific literature to justify your choice?

Reference no: EM133124450

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