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While separating the components of a mixture of Blue Dextran, cytochrome c, as well as DNP-glycine by ion exchange chromatography, the eluent is changed from 0.1 M acetate buffer to 1.0 M acetate buffer once the first colored component begins to exit the column. Explain why is the eluent changed from low salt concentration to high salt concentration? Define the order in which the components would elute from the CM Sephadex column.
the pressure on a balloon holding 411 ml of an ideal gas is increased from 211 torr to 1.00 atm. what is the new
an element has 3 stable isotopes with natural abundances of 11 17 and 72. the masses of those isotopes are 19.9 21.9
Hydrocyanic acid (HCN) is a weak acid with Ka = 4.9 x 10-10. The pH of a solution that contains equal numbers of moles of HCN and KCN is closest to
Methane and hydrogen sulfide form when hydrogen reacts with carbon disulfide. Identify the excess reagant and calucate how much remains after 36 L of H2 reacts with 12 L of CS2.
What is the average current passing through a solution of NiSO4 if 1.25 g of Ni were deposited in 4.07 hours? i = A
The resultant mixture is then evaporated in a kettle to produce a jam contain- ing 67 wt % soluble solids
a 6.0 g sample of cuh2o4so4xh2o molar mass 249.68 gmol is dissolved in deionized water. if an excess of ammonia is
Verify the following statements (a) One part per million corresponds to one minute in two years, or a single penny in $10,000.
what in your opinion is the difference between a decentralized architecture and a centralized architecture? what is an
When the two cylinders are connected and the gases mixed, the pressure in each cylinder becomes 8.71 atm. What is the volume of cylinder B?
the first ionization energy of bismuth is erm i1 703 rm kjmol. what is the longest possible wavelength of light that
A 100.0-L flask contains a mixture of NO2 gas and CO gas. If the concentration of NO2 is 5.5 × 105 ppm, and the total pressure is 790 torr, what is the partial pressure of CO in the flask?
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