Explain why is it important to allow the level of the liquid

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Once the chromatographic column has been prepared, why is it important to allow the level of the liquid in the column to drop to the level of the silica gel before applying the compound to be separated?

Also, Arrange the following in order of increasing Rf on thin-layer chromatography: acetic acid, acetaldehyde, 2-octanone, decane, and 1-butanol.

Reference no: EM13499690

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