Explain which proteins will bind tightly to cm sephadex

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Reference no: EM13270679

a) Would size exclusion chromatography be an effective way to separate Protein D from the other proteins? Why or why not?
b) Which protein(s) will bind tightly to CM Sephadex (a cation exchange resin) if loaded onto the column in a sample buffer whose pH is 7.0?

Protein A - pI of 7.4 and molar mass of 82,000
Protein B - 3.8, 21500
Protein C - 7.8, 23000
Protein D - 7.8, 22000

Reference no: EM13270679

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