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1. bring 5ml of bromophenol blue to your work station. obtain a P20 Pipetman and record below the digits you see in the window on the side. What volume is the micropipette set to deliver?
2. Set your P 20 to deliver 2ml . Using the tiny bead of dye you just placed onto the Paraflim, transfer dye to a second piece of Parafilm using 2ml increments- this requires pipetting a total of 5 times.
As you draw up the last bit of dye the 5th time around, look closely at the fluid in the pipette tip- does it contain 2 ml? I f not, speculate on where you may have lost some of the dye's volume?
There are several reagents that can be used to effect addition to a double bond including: acid and water, oxymercuration-demercuration reagents, and hydroboration-oxidation reagents
Calculate the solubility in g/L of MgCO3 in the following solutions. (a) pure water (b) 0.0010 M Na2CO3 (Use the quadratic equation.) Calculate the molar solubility of AgBr in a 3.0 M NH3 solution.
Draw the structure of the isomer of C4H9Cl that is least reactive in an SN2 reaction. Do not consider stereochemistry.
Formic acid, HFor, has a Ka value equal to about 1.8x10-4. A student is asked to prepare a buffer having a pH of 3.40 from a .10M HFor and a .10M NaFor solution. How many milliliters of the NaFor solution should she add to 20mL of the .10M HFor to..
A critical reaction in the production of energy to do work or drive chemical reactions in biological systems is the hydrolysis of adenosine triphosphate
Tris is widely used in a biochemical laboratory. Its acid dissociation constant is 8.32 x 10^-9. You have available at your lab bench a 0.1 M solution of Tris in its protonated form, 0.1 M solution of HCL and NaOH,
Would the pH of a 0.10 M formic acid (HCOOH) solution be higher or lower than the pH of a solution containing 0.10 M formic acid and 0.10 M sodium formate (HCOONa)?
The half-life of radon-222 is 3.8 days. How much of a 100 g sample is left after 15.2 days?
Next calculate molar internal energy and molar heat capacity assuming vibrational energy levels are not significantly populated at 298 K and do not contribute to molar heat capacity and molar internal energy. Calculate percent error for each molar..
Predict the rate of change in the concentration of N2O over this time interval. In other words, what is dt [N2O]/ dt?
Explain the difference between the Bohr model for the hydrogen atom and the quantum-mechanical model.
Where would we predict benzene to absorb if we treated the pi-electrons as particles on a ring (radius 1.39 A)? What is the % error? I'll give you a hint.
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