Explain what order will the aminoo acids elute

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Reference no: EM13291446

A mixture of Phe (pI 5.5), Thr (pI 6.5), Asp (pI 3.0), His (pI 7.6), and Lys (pI 9.8) at pH 9.8 is loaded on an anion-exchange column and then eluted with a pH gradient of lowering pH. what order will the aminoo acids elute? Explain.

Reference no: EM13291446

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Explain what order will the aminoo acids elute : a mixture of Phe (pI 5.5), Thr (pI 6.5), Asp (pI 3.0), His (pI 7.6), and Lys (pI 9.8) at pH 9.8 is loaded on an anion-exchange column and then eluted with a pH gradient of lowering pH. what order will the aminoo acids elute? Explain.

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