Explain what is the ratio of tris base to tris acid

Reference no: EM13668748

An enzyme catalyzed reaction is carried out in a 50mL solution containing 0.1 M TRIS buffer. The pH of the reaction mixture at the start was 8.0. As a result of the reaction, 00002 mole of H+ were produced. Explain what is the ratio of TRIS base to TRIS acid at the start of the experiment?

Explain what is the ratio at the end of the experiment?
Explain what is the final pH?

Reference no: EM13668748

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