Explain what is the protein concentration of the undiluted

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Reference no: EM13669978

Aliquots of a 0.5-mg/mL standard of BSA are utilized to construct a standard curve for the Bradford protein assay. The tubes contain the subsequent amounts of the BSA solution: 0, 20, 40, 60, 80, and 100 μL. The corresponding absorbencies subsequent to adding Bradford reagent are 0, 0.05, 0.09, 0.14, 0.19 and 0.22. If you take 20 μL of an unknown as well as add 80 μL of water, mix, take 10 μL of the mixture and add to Bradford reagent as well as see an absorbance of 0.08, Explain what is the protein concentration of the undiluted unknown?

Reference no: EM13669978

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