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A 50 mL crude extract of skeletal muscle contains 32 mg of protein per mL. Ten microliter of the extract catalyzes a reaction at a rate of 0.14 micromol product formed per minute. The extract was frationated by ammonium sulfate precipitation, and the fraction precipitating between 20% and 40% saturation was redissolved in 10 mL. This soluction contains 50 mg/mL protein. Ten microliters of this purified faction catalyzes the reation at a rate of 0.65 micromol/min.
a) what is the degree of purification (fold purification)?
b) what is the percent yield of the enzyme recovered in the purified fraction?
Fish produce glycerol to survive the freezing waters of the Arctic. Determine the degree to which the freezing point of blood and other biological fluids is lowered with the help of glycerol. What is the ideal concentration range of glycerol requi..
The heat of formation of KCl(s) is -436.7 kJ/mol and the heat of formation of KCl(aq, 1 m) is -419.5 kJ/mol. Determine the heat of solution of KCl.
how many years would it take for a 10-gram sample of U-238 to decrease to 2.5 grams?
Using a stock solution of 95.4ppm MgCO3, you are directed by your instructor to make calibration standards of 1.0, 7.5, and12.0 ppm. If you use 100mL volumetric flasks to make the solutions, what volume of stock solution will you require for each ..
Initially, the left compartment contains 1.0mol of N2, the middle contains 3.0 mol of O2, and the right contains 4.0 mol of H2 at 298 K. Calculate the Gibbs energy and entropy of mixing when the partitions are removed.
a positive ion ESI mass spectrum of a protein showed a peak at m/z = 1001 resulting from the adition of 10 protons. what is the actual mass of the uncharged protein?
A box contains a mixture of small copper spheres and small lead spheres. The total volume of both metals is measured by the displacement of water to be 427cm^3 and the total mass is 4.36 kg . What percentage of the spheres are copper?
A compound, C5H12O, has an 1H NMR spectrum showing peaks at delta 0.9 (6 H, triplet), 1.5 (4 H, quintet), and 1.9 (1 H, singlet), and 3.4 (1 H, multiplet).Draw this molecule.
Calculate the concentration of a NaOH solution if 0.1379 g of succinic acid (H2C4H4O4, 118.09 g/mol) requires 12.4 mL of NaOH solution
The atomic mass of element X is 33.42 amu. A 15.05-g sample of X combines with 79.32 g of another element Y to form a compound XY. Calculate the atomic mass of Y.
If 8686 C of charge is passed through molten magnesium chloride, calculate the number of moles of Mg(l) produced
What is the mechanism for the trans-esterification reaction of acetylsalicylic acid and methanol into methyl salicylate
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