Explain what is the percent yield of the enzyme recovered

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Reference no: EM13269709

A 50 mL crude extract of skeletal muscle contains 32 mg of protein per mL. Ten microliter of the extract catalyzes a reaction at a rate of 0.14 micromol product formed per minute. The extract was frationated by ammonium sulfate precipitation, and the fraction precipitating between 20% and 40% saturation was redissolved in 10 mL. This soluction contains 50 mg/mL protein. Ten microliters of this purified faction catalyzes the reation at a rate of 0.65 micromol/min.

a) what is the degree of purification (fold purification)?

b) what is the percent yield of the enzyme recovered in the purified fraction?

Reference no: EM13269709

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