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1. Explain what is affinity chromatography?
2. Explain how does a protein a column work in the purification of an antibody?
3. Explain what is the need for equilibration of a protein A column with the binding buffer?
4. Explain what will happen if you do not wash the column before elution?
5. Define what is the principle behind elution of an antibody from a protein a column?
Why does "ordered" protein have a smaller ensemble of wsubf than the unfolded protein wsubu and therefore a lower entropy
Explain what is the mole fraction of methane in the mixture. Assume that the water produced by the combustion is in the gaseous state.
the dipole moment mu of hydrogen bromide hbr a polar covalent molecule 0.790 d debye and its percent ionic character
Calculate the volume of dry CO2 produced at body temperature (37 degrees Celcius) and 0.970 atm when 24.5g of glucose is consumed in this reaction.
nh4no3 decomposes according to the equation...2nh4no3 s ---gt 2n2 s o2g 4h2o gwhat pressure in torr will develop in a
Calculate the bond order for one carbon-carbon bond in benzene molecule, taking a and pi bonds
If you have 340.0 mL of water at 25.00 °C and add 120.0 mL of water at 95.00 °C, what is the final temperature of the mixture. Use 1.00 g/mL as the density of water.
Given that the energy of combustion (?E) for palmitic is -39.2 kJ/g, calculate the heat capacity of the bomb calorimeter. Make sure you enter kJ/C for your unit behind the number.
Work function. A photon with 250 nm wavelength hits an Au surface (work function 5.28 eV). What's the kinetic energy (in eV) of the ejected electron? What's its speed (in km/s and mph)
A mixture is prepared using 25.00 mL of 0.08000 M aniline, 25.00 mL of 0.06000 M sulfanilic acid and 1.00 mL of 1.230 × 10-4 M HIn and then diluting to 100.0mL, where HIn is the protonated indicator. The absorbance measured at 550nm in a 5.000 cm ..
The compound has a molecular formula of C7H14O2. Draw the structure from the following NMR data: Ha=1H 1.2 ppm Hb=3H 1.3 ppm Hc=4H 4.1 ppm
The ester ethyl p-nitrobenzoate is more reactive towards nucleophilic acyl substitution than ethyl p-methyoxybenzoate. Explain the reactivity difference
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