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Explain what in vitro selection is

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Reference no: EM131127218

1. Derive θ = [L]/ ([L] + Kd) where θ is the fraction bound, L is ligand, P is protein, and Kd is dissociation constant.
(hint: the protein has only one binding site)

2. Derive Hill equation. (hint: the protein has n binding sites)

3. Derive the Michalis - Menton equation using the steady-state approximation. Show all your steps.

4. Use a program like Excel to draw a plot (hint: x axis: 1/S, y axis: 1/v ) and calculate the Km and vmax for the
following data.

2190_data1.jpg

5. Draw a plot and calculate the Km and vmax using the data in column vA. Then calculate Km and vmax using the
data in column vB. (hint: A is for substrates only, B is for substrate with inhibitor).

1837_data2.jpg

6. For question #5 B, what type of inhibitor was used? Explain by drawing the potential structure of enzyme and
where the inhibitor is binding.

7. Explain what "in vitro selection" is and what it is useful for.

Reference no: EM131127218

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