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Tris is a commonly used buffer in biochemistry. It works well in the neutral to slightly basic pH range. A working solution of Tris-HCl buffer was made by mixing equal volumes of Tris base solution (made by dissolving 0.1454 g of Tris base in 50 mL of deionized water) with HCl solution (made by adding deionized water to 4 mL of 0.1 M HCl until the volume was 50 mL). The working buffer solution was warmed to 30 degrees C and the pH of this solution was determined to be 8.03. What is the pKa of Tris under these conditions?
positron cannot penetrate matter more than a few atomic diameters but positron emission of radiotracers can be
Create a procedure of not more than 5 steps that you think will work to compare the efficiency of the light emitted by an incandescent light bulb to that emitted by a fluorescent light bulb. Make sure to mention all the variables you think you'll ..
A flask containing gaseous N2 is irradiated with 25 nm light. The irradiated moleculars of N2 can absorb the energy from the light photons. Using the following information indicate what species can from in the flask during irradiation.
The final temperature of the water and iron was 48.4oC. Assuming that the calorimeter itself absorbs a negligible amount of heat, what was the mass
How many grams of sodium lactate ( or ) should be added to 1.00 of 0.140 lactic acid ( or ) to form a buffer solution with = 4.10? Assume that no volume change occurs when the sodium lactate is added.
For a reversible reaction with a one-step mechanism, A B , the rate of the forward reaction is ratef = 3.2 x 10-6 s-1 [A] and the rate of the reverse reaction is rater = 4.6 x 10-4 s-1 [B]. What is the value of Kc for this reaction
Using a device similar to a mass spectrometer, a sample of an ancient wooden object was found to have a ratio
Consider the reaction shown below. PbCO3(s) PbO(s) + CO2(g) Calculate the equilibrium pressure of CO2 in the system at the following temperatures.
Determine a dimension characteristic of a single aluminum atom. Compare your estimate value to the tabulated atomic radius for aluminum.
Using concentrated acetic acid and solid sodium acetate, how would you prepare 10 l of 0.1 M acetate buffer at pH 4.75? The pKa for acetic acid is 4.75. What Volume of concentrated acetic acid is needed (mL)?
A compressed gas cylinder contains 1.00 x 103 g of argon gas. The pressure inside the cylinder is 2050 psi at a temperature of 18oC. How much gas remains in the cylinder if the pressure is decreased to 650psi at a temperature of 26oC
what is the solubility of CaF2 in a solution that has a equilibrium concentration of .01M EDTA. Ksp (CaF2)= 3.9 x 10^-11, Kf(CaEDTA)=5x10^10
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