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A 2500 base-pair long cDNA of unknown sequence was cloned into the MCS of a plasmid vector that has universal priming sequences adjacent to and on both sides of the MCS. On one side this is called the "forward universal priming sequence" and on the other it is called the "reverse universal priming sequence." Sanger sequencing was done using primers that are specific for each of these universal priming sequences and approximately 700 nucleotides of sequence was determined from each primer. Assuming it is not possible to obtain sequence analyses longer than 700 nucleotides, describe the steps that must be done to obtain the remainder of the sequence of the cloned cDNA.
Hint: There are two very different methods that will accomplish this. One method requires much more effort and is less reliable than the other. Either method will receive full credit.
What colour horse would you use to expose the genotype of a black trotter. Why do mosses and ferns both require free water to complete their life cycles? At what stage of the life cycle is the water required.
Which hormone causes an increased output of enzyme-rich pancreatic juice and stimulates gallbladder contraction to free bile. Build up a network design that accommodates these requirements and keeps costs down. These training environments must hold a..
What basic similarities among mammals and differences between mammals (the ingroup) and reptiles (an outgroup) help support the hypothesis that all mammals share a common ancestor.
Describing how you would test the hypothesis. Start by examining the living species around you. Ever wonder why the pollinator is attracted to the particular flower.
What is the possibility that their first child will have attached earlobes. which among the conditions or substances do ALL archaeans need for survival and reproduction.
Explain the general function of Respiratory system and describe how the Respiratory system contributes to the physiological homeostasis of human organism?
If I wanted to test the drug to see if it made mice run faster, how could I test that and what would be my control?
Make the statement that there is an equal number of all four bases (A, T, C, G) and that the sequence of bases is random, how often will a single operator be expected to appear by chance. What factors or clues lead you to this diagnosis.
If the experimental animals in a chamber were engaged in physical activity, how would this change the results of metabolism experiment.
Utilize darwin's theory of evolution by natural selection to explain how a group of ancient mammals couple be the ancestors of a major new group of mammals, the cetaceans.
Examine the nature of water and how its unique chemical properties impact water regulations in plants. Elucidate the structure and function of the leaf to water regulation.
She was hospitalized on September 17. Thereafter she developed flaccid paralysis, had loss of mental acuity, and died on October 1. Serum collected on October 2 was negative for rabies antibody, but serum collected on September 25 had a 23 titer.
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