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you have an 45kD histidine tagged bacterial protein (100% pure). you apply this pure protein to a Ni+2-agarose column. then you apply a crude mouse cell extract to the column. after extensive washing, you apply an imidazole buffer. analysis of the resultant elution on an SDS-PAGE gel indicated two proteins (45kD and 90kD) of equal coomassie blue staining.
What is the simplest explanation of why the 45kD and the 90kD proteins copurified the column?
What would be an alternative explanation?
Assume that a particular reaction evolves 239 kJ of heat and that 27 kJ of PV work is gained by the system. what are the values of delta H and delta E? Assume that a particular reaction evolves 239 kJ of heat and that 27 kJ of PV work is gained..
A solution is prepared by dissolving 297 g of sucrose (C12H22O11) in 624 g of water. What is the vapor pressure of this solution at 30°C? (The vapor pressure of water is 31.8 mmHg at 30°C.)
consider two jars. one jar contains 100 blue spheres and the other jar contains 25 each of red blue green and yellow
on't 2 and 3 have multiple answers? I think I'm only supposed to have one, though? How do you differentiate using the water solubility chart?
How much energy is required to evaporate 100.0 grams of water that is at 100.0 degrees Celsius?
36.45 mL of 0.11 M hydrochloric acid were added to 36.45 mL of 0.098 M aqueous barium hydroxide. Calculate the theoretical yield of the salt produced.
Calculate the % of acetic acid in vinegar is the mass of acetic acid is 0.104 g and the mass of the vinegar is 2.00 mL, assuming that the density of vinegar is 1.02 g/mL.
Draw a structural formula that you would predict in aspirin in each of the following biological environments. a. Esophagus pH 6.8
245.0 nm ultraviolet light strikes a metal plate and ejects electrons having kinetic energy E = 1.79 eV. Calculate the binding energy of an electron at the metal surface.
Find the absolute pressure [kPa] if the gage pressure is 30 psig when the barometer reads 698 mm Hg.
Sterling Silver is 92.5% silver by mass. How many lbs of silver are there is a 45.0 Mg piece of sterling silver?
Show the reaction equation for when sodium carbonate is added to acetic acid Show the reaction equation of acetic acid and dilute sulphuric acid. Potassium manganate is also added.
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