Explain the simplest explanation of why the 45kd

Reference no: EM13646607

you have an 45kD histidine tagged bacterial protein (100% pure). you apply this pure protein to a Ni+2-agarose column. then you apply a crude mouse cell extract to the column. after extensive washing, you apply an imidazole buffer. analysis of the resultant elution on an SDS-PAGE gel indicated two proteins (45kD and 90kD) of equal coomassie blue staining.

What is the simplest explanation of why the 45kD and the 90kD proteins copurified the column?

What would be an alternative explanation?

What control experiment could you perform to distinguish between the two possible conclusions you stated above?

Reference no: EM13646607

Questions Cloud

Define acid solution would require more volume to neutralize : You have separate solutions of HCl and H2SO4 with the same concentrations in terms of molarity. You wish to neutralize a solution of NaOH. Which acid solution would require more volume (in mL) to neutralize the base. Why

What is the initial velocity of the second stone : A mountain climber stands at the top of a 50m cliff hanging overa calm pool of water. What is the initial velocity of the second stone when it is thrown

Fiber insulator separating the coils-calculate the resistanc : A certain resistor is made with a 53.0 mlength of fine copper wire, 4.76*10-2 mm in diameter, wound onto acylindrical form and having a fiber insulator separating the coils. Calculate the resistance. (The resistivity of copper is1.72*10-8 Ω-m.)

Estimate the elastic modulus of this tendon : A 12 cm long animal tendon was found tostretch 3.9 mm by a force of13.9 N. Calculate the elastic modulus of this tendon

Explain the simplest explanation of why the 45kd : What is the simplest explanation of why the 45kD and the 90kD proteins copurified the column. What would be an alternative explanation

Estimate what is the stress within the girder : A sign (mass 1900 kg) hangs from the endof a vertical steel girder with a cross-sectional area of0.18 m2. (Ignore the mass ofthe girder itself.) What is the stress within the girder

State the shrodinger equation for the system : Consider a particle of mass, m, moving in a one dimensional system between x=a and x=b with the potential energy, V(x) = 1+(hbar squared/mx^2).

Determine the turns ratio of the transformer : The rechargeable batteries for a laptop computer need a muchsmaller voltage than what a wall socket provides. Therefore, atransformer is plugged into the wall socket and produces then ecessary voltage for charging the batteries. The batteries arer..

Estimate the volume flow rate of the blood : Suppose that blood flows through the aorta with a speed of0.46 m/s. The cross-sectional area ofthe aorta is 1.8 multiplied by 10-4 m2. Find the volume flow rate of the blood

User Account

All Pages