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Explain the reactivation half-lives

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Reference no: EM133240820

Weigle J.J. 1953. Induction of mutations in a bacterial virus. PNAS. 39. 628-636

Based on the article

Questions 

  1. How were the data for Figure 1 obtained i.e. what were the steps involved?
  2. What is a possible explanation for the differing "reactivation half-lives" for bacteria stored at different temperatures?
  3. From your current perspective of repair pathways, why is H2O2 not able to induce the damage within the cell that UV light is able to do?
  4. What were the controls on page 630 of plating irradiated or non-irradiated phage with supernatant, and the two-step adsorption protocol (same paragraph) testing for?
  5. Why do you think reactivation did not work for T2, T3 or T5 bacteriophages?
  6. Discuss the ramifications of the observations that a) no mutants are found among survivors of UV'd phage plated on normal bacteria; and b) no mutants are found among survivors of non-UV'd phage plated on UV'd bacteria.
  7. What do you think is the reason that the percentage of mutants varies with the UV dose given to the phage, and with the UV dose given to the bacteria?

Reference no: EM133240820

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