Explain the rate constants ka and kb from measurements

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Reference no: EM13520028

Consider the dimerization 2A to A2, with forward rate constant ka and backward rate constant kb. (a) derive the following expression for the relaxation time in terms of the total concentration of protein [A]tot = [A] + 2[A2]: (1/tau^2) = kb^2 + 8kakb[A]tot

(b) describe the computational procedures that lead to the determination of the rate constants ka and kb from measurements of tau for different values of [A]tot

(c) use the data provided below and the procedure you outlined in part b to calculate the rate constants ka and kb, and the equilibrium constant K for formation of hydrogen-bonded dimers of 2-pyridone

Reference no: EM13520028

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