Explain the rabbit brain tissue was homogenized

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Reference no: EM13498402

Scientists are working on purifying PFK-1C from rabbit brain tissue. The rabbit brain tissue was homogenized and centrifuged to remove insoluble material. Next, the soluble preparation was loaded on top of an ATP-Sepharose column. This is an affinity column in which ATP is covalently linked to a polysaccharide bead. This sample is loaded on top of the column, washed with a low-salt buffer, followed by a wash with a high salt buffer. What is the rationale for this procedure and what would the expected elution profile look like? (Keep in mind PFK-1A Mr = 84000 dal, PFK-1B Mr = 80000dal, PFK-1C Mr=86000 dal)

Reference no: EM13498402

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