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1. Explain the purpose of incubating the nitrocellulose filter (NCF) in 5% (w/v) defatted milk powder in PBS/Tween before applying the primary antibody.
2. Could you use an alternative to the milk powder?
3. Describe how the western blot detection system utilizing an alkaline phosphatase conjugated secondary antibody and the substrate BCIP (5-bromo-4-chloro-indolyl-phosphate) works.
4. Another detection system available for western blotting involves enhanced chemiluminescence (ECL) what advantage does this offer compared to the AP-linked -BCIP method?
5. A researcher performs a routine western blot assay and is dismayed that there are no bands detectable, including the previously reliable positive control. She suspects that the transfer may have failed. Can you suggest a way of testing whether this is the problem?
6. Non-specificity is often a problem with western analysis especially when trying to detect a target protein in a complex mixture. Discuss some strategies to increase specificity.
What are the challenges with obtaining this information? How do seasons/different years affect the data collected? for macroinvertebrates as bioindicators
The allele for not having dimples (d) is recessive. If a woman (DD) and a man (Dd) have four children, how many of the children will not have dimples?
A standard curve of absorbance as a function of oxidized DPIP concentration in ug/ml gives the following equation for the trend line: Y=.5X. The reaction volume was 7ml.
Why do we want to know the answer to the question we are asking? Finally, it should end the hypothesis from your Week Two experiment, and the reasoning behind your hypothesis.
Give one example of a coupled reaction that occurs during photosynthesis or respiration
What action of the digestive system was simulated when you broke the antacid tablet into pieces?
Discuss the importance of epidemiology to public health. Provide one current example of disease outbreak
A 3500 pF air-gap capacitor is connected to a 32 V battery. If a piece of mica fills the space between the plates, how much charge will flow from the battery?
How does digestion progress once a carbohydrate reaches the small intestine, and what substances must be released from the pancreas to allow digestion to continue?
The phrase " You are what you eat" is a simple way to tell people to be mindful of what they're eating. Everything you consume basically stays in your body
Ecological succession - include what it is and the difference between primary and secondary
q1. why will someone suffering from hyponatremia a deficiency of sodium ions in blood suffer from neurological symptoms
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