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1. Explain the purpose of incubating the nitrocellulose filter (NCF) in 5% (w/v) defatted milk powder in PBS/Tween before applying the primary antibody.
2. Could you use an alternative to the milk powder?
3. Describe how the western blot detection system utilizing an alkaline phosphatase conjugated secondary antibody and the substrate BCIP (5-bromo-4-chloro-indolyl-phosphate) works.
4. Another detection system available for western blotting involves enhanced chemiluminescence (ECL) what advantage does this offer compared to the AP-linked -BCIP method?
5. A researcher performs a routine western blot assay and is dismayed that there are no bands detectable, including the previously reliable positive control. She suspects that the transfer may have failed. Can you suggest a way of testing whether this is the problem?
6. Non-specificity is often a problem with western analysis especially when trying to detect a target protein in a complex mixture. Discuss some strategies to increase specificity.
It is a very curcial concept to understand how the immune response is mounted against viruses, bacteria, protozoans and helminthes. For an effective immune response, both innate and adaptive immunity should work together.
This Project report elaborates a critical review of important elements attached to Advanced Glycated End Products (AGEs). It is very crucial to understand the process called Millard reaction.
Soil stabilization is the permanent physical and chemical alteration of soils to enhance their physical properties. Stabilization can increase the shear strength of a soil and control the shrink-swell properties.
This assignment has three parts which contains questions related to Microbiology. It contains basic principles of microscopy, staining techniques in microbiology and microbial growth in the food industry.
Lipid metabolites are often seen as key elements in cellular signaling. Is this unique? Please provide several examples of the function of lipids as key elements in signal arrays and list the biologic functions these signals affect?
Please describe how one might search for chemical structure, biologic function relationships, involving small molecular weight lipophylic compounds. Provide one example.
Write a case study which detailing a scenario of a patient being investigated in the Haematology laboratory.
The use of PCR and genetic approaches in biotechnology
Glucose oxidase is an enzyme that can be used for measurements of glucose levels by combining this reaction with an oxygen probe.
What phenotypic ratio would you get if you crossed a white mouse and a heterozygous brown mouse?
Prepare an essay on nosocomial infection.
To increase the awareness of monitoring and recording the blood pressure of patients and practice measuring blood pressure in a safe environment.
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