Explain the purification of a protein from bovine live

Reference no: EM13163584

1) Describe in detail(ex:individual resins used, etc) the purification of a protein from bovine live that has the following characteristics: Molecular weight of 86,500, pI= 8 and precipitates out of solution at 30% ammonium sulfate 2) Which of the following polypeptides is most likely to form an alpha helix? Explain? a) Cys-Arg-Ala-Gly-Asn-Arg-Lys-Ile-Leu-Glu-Thr-Tyr b) Ser-Glu-Asp-Asn-Phe-Gly-Ala-Pro-Lys-Ser-Ille-Leu-Trp c) Gln-Lys-Ala-Ser-Val-Glu-Met-Ala-Val-Arg-Asn-Ser-Gly- 3) What is the pH of a 1L solution of 0.35 M acetic acid(ka acetic acid= 1.74x10^-3) pKa of acetic acid_ 4.76? Also if you accidentally spilled 50ml of water into this solution, what would the pH be? 4) which has the greater entropy a polymeric molecule or a mixture of its constituent monomers? explain? 5) which of the following are never spontaneous? Explain a) a reaction that occurs with any size decrease in enthalpy and any size increase in entropy b) a reaction that occurs with a small increase in enthalpy and large increase in entropy c) a reaction that occurs with a large decrease in enthalpy and a small decrease in entropy d) a reaction that occurs with any size increase in enthalpy and any size decrease in entropy?

Reference no: EM13163584

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