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An ampicillin-resistant, tetracycline-resistant plasmid, is cleaved with the enzyme PstI, which cleaves within the ampicillin resistance gene. The cut plasmid is mixed with PstI digested Arabidopsis DNA and the mixture is used to transform antibiotic sensitive E. coli cells. The E. coli cells were plated on a medium containing ampicillin or tetracycline. a) On which plate would you expect to see growth of bacteria containing plasmids with Arabidopsis DNA insert? Explain why? b) Suppose you plated the E. coli cells, mentioned above, on a medium with both antibiotics, and a number of colonies were observed. How can you explain the presence of colonies that are resistant to both antibiotics.
It is a very curcial concept to understand how the immune response is mounted against viruses, bacteria, protozoans and helminthes. For an effective immune response, both innate and adaptive immunity should work together.
This Project report elaborates a critical review of important elements attached to Advanced Glycated End Products (AGEs). It is very crucial to understand the process called Millard reaction.
Soil stabilization is the permanent physical and chemical alteration of soils to enhance their physical properties. Stabilization can increase the shear strength of a soil and control the shrink-swell properties.
This assignment has three parts which contains questions related to Microbiology. It contains basic principles of microscopy, staining techniques in microbiology and microbial growth in the food industry.
Lipid metabolites are often seen as key elements in cellular signaling. Is this unique? Please provide several examples of the function of lipids as key elements in signal arrays and list the biologic functions these signals affect?
Please describe how one might search for chemical structure, biologic function relationships, involving small molecular weight lipophylic compounds. Provide one example.
Write a case study which detailing a scenario of a patient being investigated in the Haematology laboratory.
The use of PCR and genetic approaches in biotechnology
Glucose oxidase is an enzyme that can be used for measurements of glucose levels by combining this reaction with an oxygen probe.
What phenotypic ratio would you get if you crossed a white mouse and a heterozygous brown mouse?
Prepare an essay on nosocomial infection.
To increase the awareness of monitoring and recording the blood pressure of patients and practice measuring blood pressure in a safe environment.
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