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An ampicillin-resistant, tetracycline-resistant plasmid, is cleaved with the enzyme PstI, which cleaves within the ampicillin resistance gene. The cut plasmid is mixed with PstI digested Arabidopsis DNA and the mixture is used to transform antibiotic sensitive E. coli cells. The E. coli cells were plated on a medium containing ampicillin or tetracycline. a) On which plate would you expect to see growth of bacteria containing plasmids with Arabidopsis DNA insert? Explain why? b) Suppose you plated the E. coli cells, mentioned above, on a medium with both antibiotics, and a number of colonies were observed. How can you explain the presence of colonies that are resistant to both antibiotics.
How many mls of 0.1 M NaOH would be needed to neutralize completely 250 ml of the 0.2M HA solution?
ER signal sequence contains multiple lysine and arginine residues. The plasma membrane is a sorting station of inward endocytic pathway. RabGTPases and tethering proteins are the major specificity determinant of vesicle transport system.
What happens to a cell with low ATP concentrations? Give step by step process that occurs within the cell.
Cystic fibrosis (CF) is a common genetic disease that resultsfrom a defect in a chloride transport protein. Patients afflictedwith CF have thickened mucus in the lungs, pancreas, and sweatglands.
This is the F1 generation in the Mendelian terms. Whether this generation homozygous or heterozygous? What color would its beans be (in other words, what is its phenotype)?
The PCR-derived DNA fragment used an insert in pUC19 was amplified by thenty-five cycles. Suppose that the PCR amplification process and restriction digestion process are 100% efficient.
Where are the proteins of the nucleolus synthesized?
Describe the pathway of conduction from the atria to the ventricles and correlate this conduction with ECG waves.
why are distributed rehearsals so effective? what characteristics of a living thing does mononucleosis What is the fundamental difference in how bacterial and eukaryotic genes are regulated?
In any given time the amount of cells undergoing mitosis, even in the cancer cells, are not more than 5% of the total cells. Why do you think that drugs that interferes with mitosis are able to shrink or eliminate tumors?
If a DNA molecule of 50 base pairs contain 20 cytosine bases (C), how many thymine bases will it have?
This one requires that you think about what limits the respiration rates in states IV, III, and in uncoupled mitochondria. Which of the following situations should produce the fastest rate of respiration? Let the substrate be the same in each case..
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