Explain the preparation of tris buffer

Reference no: EM13148924

Tris is widely used in a biochemical laboratory. Its acid dissociation constant is 8.32 x 10^-9. You have available at your lab bench a 0.1 M solution of Tris in its protonated form, 0.1 M solution of HCL and NaOH, and ample distilled water. Describe the preparation of 1L solution of 0.02M Tris buffer, PH 7.8. Please don't just write numbers.. explain it.

Reference no: EM13148924

Questions Cloud

How many moles of o2 are consumed : In the reaction P4 + 5 O2 → 2 P2O5, how many moles of O2 are consumed each time 0.5 moles of P2O5 form?

Determine the flexible budget variances : Determine the flexible budget variances. Provide another name for the fixed cost flexible budget variance. Indicate whether the variances are favorable (F) or unfavorable (U).

Design a mean chart and a range chart : The measurements (in millimeters) for the period from 8:00 a.m. to 10:30 a.m. follow. Use Microsoft Excel to design a mean chart and a range chart. Is the process in control or out of control?

What is the price of gasoline for this analysis : If you drive 20,000 miles per year and the price of gasoline for this analysis is assumed to be $4.00 per gallon, which model should you purchase?

Explain the preparation of tris buffer : Describe the preparation of 1L solution of 0.02M Tris buffer, PH 7.8. Please don't just write numbers.. explain it.

What mass (in grams) of nh4cl is needed : What mass (in grams) of NH4Cl is needed to prepare 450mL of 0.251 M NH4Cl solution?

What is a proximate cause of this increased urination : A female cat in heat urinates more often and in many places. Male cats are attracted to the urine depostits. What is a proximate cause of this increased urination?

Probability selected student is either caucasian or black : What is the probability that a randomly selected student is either Caucasian or Black? What is the probability that the selected student is not a member of either of the two largest categories?

Conventional measure of unemployment rate is what percent : An economy has 110,000,000 people employed 8,000,000 unemployed, and 4,000,000 discourage workers. The conventional measure of the unemployment rate is what percent?

User Account

All Pages