Explain the plate was removed from the developing chamber

Reference no: EM13538660

A TLC plate showed two spots with Rf values of 0.25 and 0.26. The plate was removed from the developing chamber, the residual solvent was allowed to evaporate from the plate, and then the plate was returned to the developing chamber. What would you expect to see after the second development was complete?

Reference no: EM13538660

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