Reference no: EM13180720
Pure solid NaH2PO4 is dissolved in distilled water, making 100.0ml of solution. 10 ml of this solution is diluted to 100.00 ml to prepare the orignal phosphare standard solution. Three working standard solution are made from this by pipetting into 0.8 ml, 1.5 ml and 3.0 ml of the orignal standard solution into 100.0 ml volumetric flasks. Acid and molybdate reagent are added and the solution are diluted to 100.0 ml. phosphate in the soft drink. You may assume that all these absorbance measurements have already been corrected for any blank absorbance. The absorbance of each is measured in the spectrophotometer. A 10.0 ml volume of soft drink is treated with acid and reagent and also diluted to 100.00 ml. Its absorbance is also measured. From the following data, plot a calibration line (absorbance vs. concentration), determine its slope and calculate the concentration of phospate in the original soft drink(mM).
Data given:
Mass of NaH2PO4 (mg) -- 536.4
Absorbance standard 1 (0.8 ml) -- 0.1973
Standard 2 (1.5 ml) --- 0.3701
Standard 3 (3.0 ml) --- 0.7402
sample (10.0 ml) -- 0.2230
Please help me with this calculation:
Concentration of orignal phosphate standard (mM)
concentration of standard 1(mM)
concentration of standard 2(mM)
concentration of standard 3(mM)
Slope of calibration line
calculate the concentration of phospate in the original soft drink(mM).
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