Explain the mobile phases added in order of increasing

Reference no: EM13223169

I am performing a gradient elution and are instructed to begin with a low polarity solvent (pet ether) and continue to increase solvent polarity with TBME. Why are the mobile phases added in order of increasing polarity rather than in the order of decreasing polarity?

Reference no: EM13223169

Questions Cloud

Depict the skeletal structure of the major organic product : Draw the skeletal structure of the major organic product resulting from the following reaction. (All hydrogen atoms are implied, but any deuterium isotopes must be shown where appropriate.)

Explain what is its temperature measured in kelvin : At room temperature a beaker of liquid measures 26 degree C. What is its temperature measured in Kelvin?

Health insurance impose substantial negative externalities : Individuals without health insurance impose substantial negative externalities on those who do. In a paragraph, list some of these externalities and briefly describe their signficance.

Describe what is the optimal price for a suit : Consider a firm with market power that sells suits. The firm has two types of customers, A and B. There are an equal number of customers of each type. Type-A customers are willing to pay up to $100 for a coat and up to $50 for pants.

Explain the mobile phases added in order of increasing : Why are the mobile phases added in order of increasing polarity rather than in the order of decreasing polarity?

What is the economic order quantity : Calculate the annual inventory holding costs based on the average inventory level and annual holding cost per box.

Explain ethyl acetate and hexanes : There is a mixture that is soluble only in methylene chloride. I wanna run the column in 10% Ethyl acetate/hexanes. Which method of column loading should I use in this case and why?

Problem - newsboy model : How many cakes with Devilicious frosting should Betty prepare daily in the morning if her objective is to maximize expected profit? What is the associated expected profit and what is the associated expected profit?

How much should the store charge for an annual membership : A local video store estimates that their average customer's demand per year is P = 3.5 - 0.5Q (all customers are identical), and knows that the marginal cost of each rental is $0.5. How much should the store charge for an annual membership

User Account

All Pages