Explain the mobile phase to ensure complete ionization

Reference no: EM13249933

HPLC analysis of basic analytes is better to perform at low pH when they are fully ionized (2 pH units lower than analyte pKa). For the analyte with pka= 5.6 and 60/40 acetonitrile/water eluent composition what should be the pH of an aqueous portion of the mobile phase to ensure complete ionization

Reference no: EM13249933

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