Explain the membrane of the lysosomes doesn''t rupture

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Reference no: EM13681041

Presume the major solutes in intact lysosomes a cellular organelle are KCI (0.1 M) and NaCl (0.03 M). We wish to isolate lysosomes from intact cells in our labs, such that the membrane of the lysosomes doesn't rupture. Presume the experiment is performed at room temperature 25 degrees C, what concentration of sucrose must we use in the extracting solution to make sure the lysosomes don't rupture with the knowledge that sucrose doesn't dissociate?

Reference no: EM13681041

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