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You have recently been hired by a research team that studies cystic fibrosis (CF), a fatal disorder that clogs the lungs and other organs with a viscous, sticky mucous that interferes with breathing and digestion. The leader of your research team has informed you that a major fraction of the mucous in CF patients consists of filamentous actin. His hypothesis is that this actin may play a key role in interfering with breathing and digestion in CF patients. In order to test your laboratory skills and knowledge he decides to give you a quiz.
Your first assignment is to assemble actin monomers into filaments. In a test tube you mix actin monomers with KCl, MgCl2, and ATP and then measure filament assembly
Your boss immediately notices that there is a lag phase before actin filaments can be detected and that the "%actin in filaments" plateaus at less than 100%.
Explain the lag phase and why the percent of actin in filaments peaks at a value less than 100%
Assume your supervisor has found a new test kit that she wants to start using. She has asked you to validate the kit. Please explain some tests that might be needed?
Normal leg size, characteristic of the Kerry type of cattle, is produced by the homozygous genotype dd. Short-legged Dexter type cattle possess the heterozygous genotype Dd.
If you wanted 10mL of a 1mM nitrophenol solution and you have on hand a 50mM nitrophenol solution, how would you make it. If you wanted 100mL of a 0.1M acetic acid solution, how much 17.4M acetic acid and how much water do you need to add to make t..
If iodine were not available, how would you determine whether starch hydrolysis had occurred? Some may see starch agar plate as an answer, however, isn't that already being used to grow the bacteria?
A technician wearing a circular metal band on his wrist moves his hand (from a region where the magnetic field is zero) into a uniform magnetic field of magnitude.
Explain the structures the dye passed through in order to get there and discuss and define the term transpiration-pull.
What does this show about the relation between voluntary and involuntary reflex movement. If the bacteriorhodopsin molecules were randomly oriented, would you expect ATP to be synthesized? Give details about the answers.
The protostome developmental sequence arose just once in evolutionary history, resulting in two main subgroups-Lophotrochozoa and Ecdysozoa. What does this finding suggest?
Many organisms possessing many different lipases produce positive results on Tributyrin Agar. Is the inability of this medium to distinguish between these different enzymes a weakness in its specificity or its sensitivity?
Define a genetic mutation and state the types of genetic mutations. Discuss the effects of genetic mutations.
Suppose a cross has been made with maize between a plant with colored, nonshrunken kernals (C/C Sh/Sh) and a plant with colorless, shrunken kernels (c/c sh/sh).
In cattle, coat color may be white, black, or black and white spotted. When true breeding white cattle are crossed with true-breeding black stock, the F1 is all solid white.
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