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Explain the function of aminoacyl-tRNA-synthetase and why it is important that this enzyme is perfectly accurate in its recognition of tRNAs and amino acids.
Nocodazole reversibly inhibits microtubule polymerization, which is essential for formation of the mitotic spindle. By treating a population of mammalian cells with nocodazole for a time and then washing it out of the medium.
Oxygen is the final electron acceptor of this pathway. Glycolysis is the only energy yielding step of this pathway.
Assume you are working to characterize a novel protein in mice. Analysis shows that high levels of the primary RNA transcript that codes for the protein are found in tissue from the brain.
Which organelle generates oxygen? Which organelle consumes oxygen? Explain why are viruses not considered to be living?
Sanger-based sequencing needs very specific DNA and primer concentrations for optimal performance. For template DNA like plasmids, DNA needs to be at a concentration of 50ng/ul in 10 ul.
Assume you have made several dilutions of p nitrophenol and are reading the absorbance of each sample on a spectrophotometer.
What effects are produced by an enzyme on the general reaction below? The rate constant for the forward reaction (K1) increases
Consider any natural populations that might be out of Hardy-Weinberg equilibrium? You need not to test this with statistics. Just name the species and explain the population.
Assume you are provided a solution of 0.1 M NaCl. What is the concentration of that solution in mg/ml? In %w/v?
Consider a human population that is similar to the ideal Hardy-Weinberg population in that it is very large and generally random-mating. Although mutations occur, they alone do not lead to great changes in allele frequencies.
Explain the development of Anabaena heterocysts. Include the names of important structures and compounds.
Every cell in the human body has two alleles that condense into single chromosomes held together through a centromere. These sister chromaatids replicate and pair with newly made homologous chromosomes.
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