Reference no: EM13237986
You are interested in purifying a sampe containing the protein alcohol dehydrogenase obtained from horse liver; however the sample also contains a second protein, catalase. These two proteins have the following transport properties:
Catalases; s (s)=11.3×10-13, D (m2s-1)=4.1×10-11, V (cm3g-1)=0.715
Alcohol dehydrogenase; s (s)=4.88×10-11, D (m2s-1)=6.5×10-11, V (cm3g-1)=0.751
a)Determine the molecular weight of catalase and alcohol dehydrogenase.
b) You have access to a centrifuge that can provide angular velocities up to 35,000 rpm. For the species you expect to travel the greatest distance in the centrifuge tube, determine the time it will take to centrifuge until a 3 cm displacement of the boundary layer occurs relative to the initial 5 cm location of the boundary layer relative to the centrifuge axis.
c) To separate the proteins, you need a separation of at least 1.5 cm between the boundary layers associated with each protein. Using your answer in part b), will it be possible to separate the proteins by centrifugation?
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