Explain the apparent vmax and km of the inhibitor

Reference no: EM13647035

The kinetics of an enzyme is measured as a function of substrate concentration in the presence and absence of 2 mM inhibitor. The data from the experiments are shown below:

a). What are the apparent Vmax and Km in the absence and in presence of the inhibitor?

Vo versus [S] plot:

without inhibitor

Vmax = 0.0629

Km=-3.269

with inhibitor

Vmax= 0.1486

Km= -3.098

Lineweaver-Burk plot

without inhibitor

Vmax=44.64

Km= -4.464

with inhibitor

Vmax=45.045

Km=-1.501

b). What kind of inhibition is it?

c). What is the KI of the inhibitor?

Reference no: EM13647035

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