Explain the aminomethane-buffer

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How would you prepare 1.000 L of a 1.000 mole L?1, pH 7.50 Tris-aka, tris(hydroxymethyl)aminomethane-buffer? You have pure Tris?HCl, {(HOCH2)3NH+,Cl?}, a pH meter, 1.00 M HCl and 1.00 M NaOH, and all of the pure water and glassware you need. Carefully go through all mathematical steps, and then describe in detail how you would make the buffer in the laboratory

Reference no: EM13162117

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