Explain solution was made basic with naoh

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The protein in a 1.2846-g sample of oat cereal was determined using the following procedure: The sample was digested with H2SO4 and the resulting solution was made basic with NaOH. This converts the nitrogen in the protein to ammonia. The NH3 that formed was distilled and collected into flask containing 50.00mL of .09552 M HCL. The resulting solution was then back titrated, requiring 37.84 mL of 0.05992 M NaOH to reach the phenolphthalein end point. Assuming that the proteins in grains has an average %w/w N of 17.54%, calculate the % w/w protein in the sample

Reference no: EM13231857

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