Explain solution of tris in its protonated

Reference no: EM13499113

Tris is widely used in a biochemical laboratory. Its acid dissociation constant is 8.32x10^-9. You have available at your lab bench a 0.1M solution of Tris in its protonated form, 0.1M solution of HCl and NaOH, and ample distilled water. Describe the preparation of a 1 L solution of 0.02M Tris buffer, pH=7.8.

Reference no: EM13499113

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