Explain separate single-stranded polynucleotides at ph

Reference no: EM13506690

An ion exchange column is used to separate single-stranded polynucleotides at pH 2. A gradient of increasing salt concentration is used for elution.

(a) Discuss which type of ion exchange would work best (anion or cation)?

(b) In this same column, would you expect triadenylic acid (ApApA) or pentaadenylic acid (ApApApApA) to elute first?

Reference no: EM13506690

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