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Explain restriction enzymes

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a. In your own words, explain restriction enzymes. b. Is DNA negatively or positively charged?

c. Toward which electrode does DNA migrate?

d. What polymer did you use to separate DNA molecules/fragments?

e. Explain how gel electrophoresis allows you to separate DNA based on the size of the molecules.

f. Within a single lane (DNA sample), which restriction fragments represent shorter pieces of DNA, and which fragments represent longer pieces of DNA?

g. Why are some bands more faintly white and some bands more boldly white?

h. Do the three subjects have the same restriction fragments? Explain your answer.

i. Compare the restriction fragments from the crime scene with those from each of the three suspects. Does the DNA from the crime scene provide evidence that one of the suspects is guilty?

j. Would you feel confident presenting your evidence at a criminal trial? i. If yes, then explain your answer in detail using your DNA fingerprinting data. ii. If no, then what would you do next as a lab technician?

Reference no: EM133124301

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