Explain maximum specific activity of a purified preparation

Reference no: EM13562056

For a single-substrate enzyme-catalyzed reaction, calculate the change in substrate concentration required to increase the rate of reaction from 10% to 90% of the limiting rate. What further change would be required to increase the rate to 95% of the limiting rate?

Under certain conditions the maximum specific activity of a purified preparation of triosephosphate isomerase was quoted as 10,000μmoles glyceraldehyde 3-phosphate transformed per mg protein per minute. Express this in terms of katal kg-1 and evaluate the turnover number of the enzyme given that its molecular weight per active site is 26,500 Da.

Reference no: EM13562056

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