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You have to run SDS-PAGE (a gel electrophoresis method for separating proteins with different MWs). You have to make the protein sample in a buffer called Laemmli buffer (LB) with DTT. The laboratory has a 5M concentration of Laemmli buffer and 1M DTT solution already ready for you. Your final protein sample should be in 1M concentration of LB buffer with 20mM DTT. Your gel sample loading capacity is 50 µL/well. Show how many µL of protein sample you will take and add with the desired volume of DTT and Laemmli buffer to make the right concentration of sample.
An automobile gasoline tank holds 36 kg of gasoline. When the gasoline burns, 95 kg of oxygen is consumed, and carbon dioxide and water are produced.
a 1.00L buffer solution is 0.250 M in HF and 0.250 M in NaF. Calculate pH of the solution after the addition of 100.0mL of 1.00M HCl. Ka for HF is 3.5x10^-4
The titration involves the formation of a stable, neutral complex: Hg2+ (aq) + 2 SCN- (aq) = Hg(SCN)2 (aq) A)How many mmoles of mercury are in the sample
where all compounds are assumed as covalent then determine which reaction must be endothermic and also explain your answer briefly?
In a titration of HNO3, you add a few drops of phenolphthalein indicator to 50.00 mL of acid in a flask. You quickly add 26.50 mL of 0.0502 M NaOH but overshoot the end point
If a sample containing 3.00 mL of nitroglycerine (density = 1.592 g/mL) is detonated, how many total moles of gas are produced?
Compute the change in temperature for 4.50 grams of Calcium chloride CaCl 2 dissolved in 90.0 mL of water H 2 O. (Assume that the calorimeter absorbs no heat )
A .692 L NaCl solution is diluted to a volume of 1.25 L and a concentration of 4.00 M. What was the initial concentration?
A sample of nitrogen gas in a 4.4-L container at a temperature of 35°C exerts a pressure of 3.7 atm. Calculate the number of moles of gas in the sample.
calculate the mass of ammonium nitrate that should be heated to obtain 100 mL of dinitrogen oxide, N2O, at 1.00 atm and 25 degrees celcius?
The average concentration of strontium ion in seawater is 8.00 mg of strontium ion per kg of seawater. What is the molarity of the strontium ion if the density of the seawater is 1.025 g/mL.
A sealed balloon is filled with 2.20 L helium at 23°C and 1.00 atm. The balloon rises to a point in the atmosphere where the pressure is 190. torr and the temperature is -35°C
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