Explain include the amounts of tris-base and edta

Reference no: EM13515057

Outline the steps necessary to properly prepare 100ml of TE buffer. Include the amounts of Tris-base and EDTA, and all steps necessary to adjust the pH, including when you add what bolumes of water. Tris-base has a molecular weight of 121 g/mol and will be very basic when it dissolves. EDTA's molecular weight is 372.2 g/mol.

TE= 10 mM Tris buffer, pH 8.0, 2mM EDTA

Reference no: EM13515057

Questions Cloud

How much of cooks net income : How much of Cook's net income did Mehan report for the year 2012?

Define which reactant is in excess from the reaction : Which reactant is in excess from the reaction of 35.0 g of hydrogen with 276 g of oxygen to form water

The corporate scandals that led to its creation : What were the corporate scandals that led to its creation

How to find out the ph of the solution after the addition : A 100.0 mL sample of 0.10 M NH3 is titrated with 0.10 M HNO3. Determine the pH of the solution after the addition of 150.0 mL of HNO3. The Kb of NH3 is 1.8 × 10-5.

Explain include the amounts of tris-base and edta : Outline the steps necessary to properly prepare 100ml of TE buffer. Include the amounts of Tris-base and EDTA, and all steps necessary to adjust the pH, including when you add what bolumes of water.

State the percent yield of a reaction : What is the percent yield of a reaction where 15.3g of oil of wintergreen is collected after 16.3g salicylic acid

Determine the net charge on the fur : A plastic bar is rubbed with fur, and the bar aquires a net charge of +8.34 uC. Determine the net charge on the fur

Explain treating a mixture of vanillin and eugenol to steam : Explain why treating a mixture of vanillin and eugenol to steam distillation would result in distillation of eugenol only

Explain the molar mass of toluene of the freezing point : What is the molar mass of toluene if 0.85 g of toleune depresses the freezing point of 100 g of benzene by 0.47 degrees C. Kf of benzene is 5.12 degrees C/m.

Reviews

Write a Review

Required(*) Message

Chemistry Questions & Answers

Steps in the mechanism for the following reaction

Show all the steps in the mechanism for the following reaction, When benzene is mixed with deuterated sulfuric acid, deuterium is slowly incorporated onto the ring. Show the mechanism for this reaction and explain how this relates the sulfonation of ..

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

Submit Assignment

User Account

All Pages