Explain hydrophobic interaction chromatography

Assignment Help Biology
Reference no: EM1398525

What is Hydrophobic interaction chromatography? and how do high salt, medium salts, and no salt buffers paly a role in the purification of GFP? I know that no salt brings polar amino acids to the surface of GFP and nopolar amino acids move inside but not sure about high or medium salt

Reference no: EM1398525

Questions Cloud

Could he be the father : A woman with blood type B has a baby with blood type O. A man she says is the baby's father has blood type A, but he denies having fathered the child. Could he be the father?
Which mode of action of an antiobiotic is least likely : which mode of action of an antiobiotic is least likely to cause side of effects in a ptient.
Explain kohlberg-s stages of moral development : Analyze your own life and try to set-up as honestly and accurately as possible where values have come from. Where might you fit in Kohlberg's Stages of Moral Development?
Find normal protein coded for by the mrna in the mixture : In an experiment, you purify a cysteine-charged tRNA and chemically alter the amino acid attached to it converting it to alanine.
Explain hydrophobic interaction chromatography : What is Hydrophobic interaction chromatography? and how do high salt, medium salts, and no salt buffers paly a role in the purification of GFP? I know that no salt brings polar amino acids to the surface of GFP
Comparing the results of two calculations of concentration : Molecular Weights as determined by SDS-PAGE & NATIVE PAGE GEL How do the two masses compare with one another as determined by the two methods?
Estimate the pressure gradient required to maintain cardiac : A patient has a stenotic mitral valve which has a reduced effective orifice area of 1.0 cm^2. The systolic ejection period is 330 milliseconds with a heart rate of 70 beats/min.
Develop a routine assay for the virus : What aspects of modern society increase the opportunities for a global pandemic with H7N9 and Describe the key molecular/cellular events in the HIV lifecycle from entry to assembly and exit from the host cell.
Which reaction releases more free energy : Which person would tend to have a higher proportion of slow-twitch than fast-twitch muscles: a weight lifter, a sprinter, or a long distance runner?

Reviews

Write a Review

Biology Questions & Answers

  When did dinosaurs and pterosaurs become extinct

When did dinosaurs and pterosaurs become extinct? Which are the only extant animals that descended directly from dinosaurs.

  Contrast the epithelium of esophagus

Contrast the epithelium of esophagus with that of the stomach. Explain why is each epithelial type best suited to the function of its respective organ?

  Non standard nucleotide x in dna

Suppose that a team of scientists exploring in the Amazon discover various novel species of bacteria that use a non-standard nucleotide "X" in DNA.

  Estimating genotype of males

A wild-type male and female  Drosophila with red eyes and full wings are crossed. Estimating genotype of males.

  Oxidative phosphorylation would slow down in mitochondria

throughput a heart attack, blood flowing to the heart muscle is interrupted by blockage of a coronary artery. How would you expect the metabolism in the heart to change?

  Comparing competitive exclusion with niche differentiation

Connell results led him to conclude that Chthamalus' realized niche is smaller than its fundamental niche because of interspecific competition with Balanus.

  Explain breast care after delivery

Explain breast care after delivery. Explain the actions of prolactin, where it is produced, and what causes its release.

  Why is the skin on worms

Discuss why is the skin on worms, the gills in fish, and the lungs in humans good epithelial surfaces for respiration. Be specific.

  Difference beween intoxication and infection

Critically discuss the difference between food intoxication and food infections. In May 2012, a young woman in Georgia was zip lining and fell into a river.

  Increasing the hydrophilicity of ldpe

Phosphonylation, a chemical surface treatment, has been proposed as a means of increasing the hydrophilicity of LDPE, and therefore increase its lubricity and reduce vascular tissue damage.

  Unattached kinetochore of a monoattached chromosome

2-types of signals have been implicated in metaphase checkpoint arrest. Think about the results of a recent experiment in which the unattached kinetochore of a monoattached chromosome was destroyed by a laser beam.

  Sensory neurons in our bodies

Some sensory neurons in our bodies generate action potentials in response to pressure or movement, allowing us to feel things through our sense of touch.

Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd